Investigation of glutamine metabolism in CHO cells by dynamic metabolic flux analysis
نویسندگان
چکیده
Background Glutamine metabolism represents one of the major targets in metabolic engineering and process optimization due to its importance as cellular energy, carbon and nitrogen source. Metabolic flux analysis represents a powerful method to investigate the physiology and metabolism of cells [1]. Classical metabolic flux analysis methods require steady state conditions. However, industrially relevant cultivation conditions, i.e. batch and fed-batch cultivations, are characterized by changing environmental conditions and metabolic shifts [2]. We used dynamic metabolic flux analysis to study the impact of glutamine availability or limitation on the physiology of CHO K1 cells capturing metabolic dynamics during batchand fed-batch cultivations.
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Towards dynamic metabolic flux analysis in CHO cell cultures.
Chinese hamster ovary (CHO) cells are the most widely used mammalian cell line for biopharmaceutical production, with a total global market approaching $100 billion per year. In the pharmaceutical industry CHO cells are grown in fed-batch culture, where cellular metabolism is characterized by high glucose and glutamine uptake rates combined with high rates of ammonium and lactate secretion. The...
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